Coding

Part:BBa_K3977000:Design

Designed by: Yilong Xu   Group: iGEM21_SCU-China   (2021-10-01)


Cas12j-D394A (or dCasΦ), for more see 2021 SCU-China wiki page result and model


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1818
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 180
    Illegal NgoMIV site found at 2178
    Illegal NgoMIV site found at 2220
    Illegal AgeI site found at 2125
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1447
    Illegal SapI site found at 1240


Design Notes

do CRISPRa and CRISPRi


Source

Biggiephage

References